Photocatalytic and electrocatalytic degradation of bisphenol A in the presence of graphene/graphene oxide-based nanocatalysts: A review.

Bisphenol A (BPA), a widely recognized endocrine disrupting compound, has been discovered in drinking water sources/finished water and domestic wastewater influent/effluent. Numerous studies have shown photocatalytic and electrocatalytic oxidation to be very effective for the removal of BPA, particularly in the addition of graphene/graphene oxide (GO)-based nanocatalysts. Nevertheless, the photocatalytic and electrocatalytic degradation of BPA in aqueous solutions has not been reviewed. Therefore, this review gives a comprehensive understanding of BPA degradation during photo-/electro-catalytic activity in the presence of graphene/GO-based nanocatalysts. Herein, this review evaluated the main photo-/electro-catalytic degradation mechanisms and pathways for BPA removal under various water quality/chemistry conditions (pH, background ions, natural organic matter, promotors, and scavengers), the physicochemical characteristics of various graphene/GO-based nanocatalysts, and various operating conditions (voltage and current). Additionally, the reusability/stability of graphene/GO-based nanocatalysts, hybrid systems combined with ozone/ultrasonic/Fenton oxidation, and prospective research areas are briefly described.

Stabilization of microbial network by co-digestion of swine manure and organic wastes.

The production of biogas from organic waste has attracted considerable interest as a solution to current energy and waste management challenges. This study explored the methane (CH4) production potential of swine manure (SM), food waste (FW), and tomato waste (TW) and the changes in the microbial community involved in the anaerobic digestion process. The results revealed that the CH4 production potentials of the four kinds of SM samples were influenced by the characteristics of SM (e.g., age and storage period). Among the four kinds of SM samples, the CH4 yield from the manure directly sampled from primiparous sows (SM3) was the highest. The CH4 yield was significantly improved when SM3 was co-digested with FW, but not with TW. The addition of SM fostered a stable CH4 production community by enhancing the interaction between methanogens and syntrophic bacteria. Furthermore, the addition of FW as a co-substrate may improve the functional redundancy structure of the methanogenesis-associated network. Overall, the characteristics of SM must be considered to achieve consistent CH4 yield efficiency from anaerobic digestion since CH4 production potentials of SM can be different. Also, the contribution of co-substrate to the synergistic relationship between methanogens and syntrophic bacteria can be considered when a co-substrate is selected in order to enhace CH4 yield from SM.

Microplastic contamination in the agricultural soil-mitigation strategies, heavy metals contamination, and impact on human health: a review.

Microplastic pollution has emerged as a critical global environmental issue due to its widespread distribution, persistence, and potential adverse effects on ecosystems and human health. Although research on microplastic pollution in aquatic environments has gained significant attention. However, a limited literature has summarized the impacts of microplastic pollution the agricultural land and human health. Therefore, In the current review, we have discussed how microplastic(s) affect the microorganisms by ingesting the microplastic present in the soil, alternatively affecting the belowground biotic and abiotic components, which further elucidates the negative effects on the above-ground properties of the crops. In addition, the consumption of these crops in the food chain revealed a potential risk to human health throughout the food chain. Moreover, microplastic pollution has the potential to induce a negative impact on agricultural production and food security by altering the physiochemical properties of the soil, microbial population, nutrient cycling, and plant growth and development. Therefore, we discussed in detail the potential hazards caused by microplastic contamination in the soil and through the consumption of food and water by humans in daily intake. Furthermore, further study is urgently required to comprehend how microplastic pollution negatively affects terrestrial ecosystems, particularly agroecosystems which drastically reduces the productivity of the crops. Our review highlights the urgent need for greater awareness, policy interventions, and technological solutions to address the emerging threat of microplastic pollution in soil and plant systems and mitigation strategies to overcome its potential impacts on human health. Based on existing studies, we have pointed out the research gaps and proposed different directions for future research.

Graphitic-carbon-nitride-hydrophilicity-dependent photocatalytic degradation of antibiotics with different log Kow.

The surface hydrophilicity of a photocatalyst is an important factor that directly influences its interactions with organic pollutants and significantly impacts its degradation. In this study, we investigated the impact of increased hydrophilicity of g-C3N4 (CN) by alkaline solvothermal treatment on the degradations of three antibiotics (oxytetracycline (OTC), oxolinic acid (OA), and sulfamethoxazole (SMX)) with different log Kow values. Scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), and Fourier-transform infrared (FT-IR) spectroscopy showed no significant differences in the morphology, crystalline structure, and surface functional groups of CN after alkaline solvothermal treatment (Nv-HPCN). However, contact angle analysis revealed that Nv-HPCN (31.8°) was more hydrophilic than CN (61.1°). To assess the hydrophilicity of the antibiotics, the log Kow values of SMX (0.77), OA (0.43), and OTC (-0.34) were measured. Nv-HPCN showed faster OTC degradation than CN, whereas the opposite pattern was observed for the degradation of OA. Scavenger tests showed that O2•- and h+ mainly contributed to the degradation of these antibiotics. Furthermore, the influences of NOM and coexisting anions on antibiotic degradation were investigated. This study thus offers perspectives on the impact of surface hydrophilicity of photocatalysts on the degradation of antibiotics.

Biochar derived from rice husk: Impact on soil enzyme and microbial dynamics, lettuce growth, and toxicity.

This study was set to investigate the effects of rice husk biochar (RHB) on soil characteristics and growth of lettuce (Lactuca sativa). A comprehensive research approach was employed to examine the effect of different RHB concentrations (i.e., 0-1.5%) on soil pH, soil enzyme activities (i.e., alkaline phosphatase, beta-glucosidase, and dehydrogenase), soil microbial community, lettuce growth, and earthworm toxicity. The results showed that, within the studied RHB concentration range, the RHB application did not have significant effects on the soil pH. However, the enzyme activities were increased with increasing RHB concentration after the 28 d-lettuce growth period. The RHB application also increased the abundances of the bacterial genera Massilia and Bacillus and fungal genus Trichocladium having the plant growth promoting abilities. Furthermore, the study revealed that the root weight and number of lettuce leaves were significantly increased in the presence of the RHB, and the growth was dependent on the RHB concentration. The improved lettuce growth can be explained by the changes in the enzyme and microbial dynamics, which have resulted from the increased nutrient availability with the RHB application. Additionally, the earthworm toxicity test indicated that the tested RHB concentrations can be safely applied to soil without any significant ecotoxicity. In conclusion, this study underscores the potential of RHB as a soil amendment with positive effects on crop growth, highlighting the utilization of agricultural byproducts to enhance soil biological quality and plant growth through biochar application.

Effect of different types and shapes of microplastics on the growth of lettuce.

The presence of microplastics in agricultural soils has emerged as a significant environmental concern due to their persistent nature. Microplastics of different properties (i.e., types, shapes, size, concentration) are present in the environment, but the studies on the effect of microplastics having different properties are limited. Thus, this study investigated the effects of different microplastics (low-density polyethylene (LDPE) fragments, polyvinyl chloride (PVC) fragments, and LDPE fiber) in soil on the growth of lettuce (Lactuca sativa L.). Pot tests were carried out to study the effect of a range of microplastic concentrations and different shapes and types of microplastics in soil on the lettuce growth. The different growth parameters such as lettuce weight, lengths, and chlorophyll contents were measured and compared. The results showed that the adverse effects of the microplastics on the lettuce growth increased with increasing microplastic concentration. The effects of LDPE fragments and fibers on the root weights and the chlorophyll contents were microplastic shape-dependent. Also, the effects of LDPE fragments and PVC fragments on the shoot and root weights and the chlorophyll contents were microplastic type-dependent. Among the three microplastics studied, LDPE fragments tend to have greater effects on the lettuce growth than the other microplastics. Overall, the results show that the effects of microplastics on different growth parameters of lettuce can be shape- and/or type-dependent. The presence of microplastics having different properties make the understanding the effects of microplastics on plants difficult, and this necessitates further studies.

Responses of earthworms exposed to low-density polyethylene microplastic fragments.

The interest in the effect of microplastics (MPs) on the terrestrial environment has been increasing recently. Different species of earthworms have been used to study the effects of MPs on different aspects of earthworm health. However, there is still a need for more studies because different studies report different effects on earthworms depending on the properties (e.g., types, shapes, sizes) of MPs in the environment and exposure conditions (e.g., exposure period). This study used Eisenia fetida as a test earthworm species to investigate the effect of different concentrations of low-density polyethylene (LDPE) MPs (≤125 µm) in soil on the growth and reproduction of earthworms. The exposure of the earthworms to the different concentrations of LDPE MPs (0-3% w/w) for 14 d and 28 d neither caused death of the earthworms nor showed significant effects on the earthworm weights in this study. The number of cocoons produced by the exposed earthworms were also similar to that of the controls (i.e., no exposure to MPs). Some previous studies reported similar results to what have been observed in this study, while some studies reported different results. On the other hand, the number of ingested MPs by the earthworms increased with increasing MPs concentrations in soil, suggesting a potential for damage to digestive tract. Also, the earthworm skin surface was damaged after exposure to MPs. The ingested MPs and the skin surface damage suggest that there is a potential for adverse effects on the earthworm growth after a longer-term exposure. Overall, the results of this study show that the effects of MPs on earthworms need to be studied using various endpoints including growth, reproduction, ingestion, and skin damage and such effects can be different depending on the exposure conditions such as MPs concentration and exposure period.

Pyrolytic Remediation and Ecotoxicity Assessment of Fuel-Oil-Contaminated Soil.

Oil-contaminated soil is a major societal problem for humans and the environment. In this study, the pyrolysis method was applied to oil-contaminated soil used as a landfill and gas station site in Korea. The removal efficiency of the main components of oil-contaminated soils, such as total petroleum hydrocarbons (TPH), polyaromatic hydrocarbons (PAHs), unresolved complex mixture (UCM), and alkylated PAHs (Alk-PAHs) were measured, and the effect of temperature, treatment time, and moisture content on pyrolysis efficiency was studied. In order to evaluate the risk of soil from which pollutants were removed through pyrolysis, integrated ecotoxicity was evaluated using Daphnia magna and Allivibrio fischeri. The chemical and biological measurements in this study include contaminants of emerging concerns (CECs). Results showed that the pyrolysis was more efficient with higher treatment temperatures, moisture content, and treatment times. In addition, toxicity was reduced by 99% after pyrolysis, and the degree of toxicity was evaluated more sensitively in Allivibrio fischeri than in Daphnia magna. This study shows that weathered oil-contaminated soil can be effectively treated in a relatively short time through pyrolysis, as well as provides information on efficient conditions and the assessment of ecotoxicity.

Fe(III)-doped activated biochar sorbents trigger mitochondrial dysfunction with oxidative stress on Daphnia magna.

This study investigates the ecotoxicological effects of the synthesized Fe(III)-doped activated biochar (FeAB) sorbents using Daphnia magna and elucidates the underline mechanism of potential oxidative stress that may be induced by the sorbent. The EC50 value was determined to be 68.8 mg L-1. The superoxide dismutase (SOD) activity of D. magna was generally inhibited and the glutathione (GSH) level was significantly reduced even at the lowest FeAB concentration used (i.e., 0.12 mg L-1). This means that the antioxidant system of D. magna can be significantly inhibited by exposure to even a small amount of FeAB. While the higher reactive oxygen species (ROS)/reactive nitrogen species (RNS) levels in the exposed samples than the control at low FeAB concentrations (i.e., <15.63 mg L-1) suggest the failure of the anti-oxidation mechanism of SOD and GSH, the lower average levels of ROS/RNS in the exposed samples than the control at relatively high concentrations (i.e., 31.25-1000 mg L-1) can be explained by the reduced ROS/RNS production due to cell damage. Furthermore, the mitochondrial complex III activities were significantly inhibited in a FeAB concentration-dependent manner. Overall, the FeAB sorbent down-regulates the antioxidant mechanism, and this, together with the inefficient mitochondria, increases the ROS generation, leading to mitochondrial dysfunction again. The potential oxidative stress of FeAB on D. manga observed in this study suggests that the environmental application of FeAB needs to adopt a method that can minimize the direct contact between FeAB and organisms.

Restoring phosphorus from water to soil: Using calcined eggshells for P adsorption and subsequent application of the adsorbent as a P fertilizer.

This study investigated the solution for two environmental issues: excess of P in water and its deficiency in soil, which is restored by transferring the adsorbed P from water into the soil using eggshell as an adsorbent. The eggshells were calcined at different temperatures to improve their adsorption capacity, and evaluated for their physical/chemical properties and P adsorption capacity. The eggshells calcined at 800 °C (CES-800) had the highest P adsorption; CaCO3 decomposed into 23.6% of CaO and 40.8% of Ca(OH)2, eluting more Ca that reacted with soluble P in water. X-ray diffraction analysis confirmed that CES-800 removed P as hydroxylapatite by reacting with Ca. Pseudo-first-order and Langmuir models suitably described the kinetic and equilibrium of P adsorption by CES-800, respectively. The maximum adsorption capacity of CES-800 was 108.2 mg g-1. As the solution pH increased from 3 to 11, the adsorption amount decreased from 99.8 mg g-1 to 62.3 mg g-1. The feasibility of CES-800 as a filter medium was assessed using real lake water under dynamic flow conditions; > 90% of P removal was achieved at 158 h, and the P adsorbed was 11.5 mg g-1. When CES-800 and P adsorbed CES-800 (P-CES-800) were applied to the soil at the studied rates, the earthworms were unaffected by toxicity, suggesting the use of both adsorbents in soil without adverse effects. The shoot fresh weight, tiller number, and total dry weight significantly increased in P-CES-800 applied rice plants compared to the control plants, indicating that P-CES-800 can be a good alternative to conventional P-fertilizer in rice cultivation.

Changes in the aquatic ecotoxicological effects of Triton X-100 after UV photodegradation.

Various spray adjuvants including surfactants are widely used in agricultural pesticide formulations, and some of them may remain in soils and waters and impose more adverse effects than active pesticide ingredients on organisms. However, previous studies are more focused on the active pesticide ingredients than the adjuvants. Thus, this study investigates the changes in toxic effects of surfactants during photodegradation, which is one way of naturally degrading contaminants in natural waters. Triton X-100, a water-soluble non-ionic surfactant, was degraded using different types of UV radiation (UVA, UVB, and UVC), and the changes in the toxic effects were determined using bioluminescent bacteria and water flea. The Triton X-100 removals were negligible with UVA within 24 h, while its removal was 81% with UVB and almost complete with UVC. The NMR spectra indicated possible molecule rearrangement after photolysis. On the other hand, the toxic effects based on the mortality of Daphnia magna and the bioluminescence of Aliivibrio fischeri increased (i.e., lower EC50 values) after photodegradation, suggesting the generation of photoproducts that are likely to have higher toxic effects or higher bioavailability. Furthermore, the sensitivities of D. magna and A. fischeri for Triton X-100 and the photodegraded Triton X-100 were different. This study suggests that the changes in the chemical composition of the Triton X-100 containing water with photodegradation can lead to changes in the relative toxic effects on different aquatic organisms. Therefore, not only the management of parent compound (i.e., Triton X-100) but also the photoproducts generated from the parent compound need to be considered when managing water environment subject to photodegradation.

Exploring reductive degradation of fluorinated pharmaceuticals using Al2O3-supported Pt-group metallic catalysts: Catalytic reactivity, reaction pathways, and toxicity assessment.

Recently, an increasing number of pharmaceutical compounds has become fluorinated. Owing to their pharmacological efficacy, the use of these fluorinated pharmaceuticals continues to grow, and they constitute 20% of the drugs on the current market. However, only a few studies have investigated the fate and transformation of these emerging contaminants in natural and engineered aquatic environments. In the present study, the H2-based reductive transformation of three fluorinated pharmaceutical compounds (levofloxacin, sitagliptin, and fluoxetine) were investigated using alumina-supported monometallic and bimetallic catalysts of the Pt-group noble metals (i.e., Ru, Rh, Pd, and Pt) under ambient temperature and pressure conditions. Degradation of all three compounds was observed with catalytic reactivity ranging from 4.0  ×  10-3 to 2.14  ×  102 L/(min·gcat), in which fluoxetine generally showed the highest reactivity, followed by sitagliptin and levofloxacin. The fluorination yields and transformation products were characterized for each fluorinated compound and three different degradation mechanisms were elucidated: 1) hydrodefluorination of C-F bond to CH bond, 2) hydrogenation of aromatic ring, and 3) reductive cleavage of CO bond from phenyl ether. Toxicity assessment using Aliivibrio fischeri showed there were no significant changes in toxicity over levofloxacin and sitagliptin degradation, suggesting the formation of no highly toxic by-products during catalytic reduction. For fluoxetine, an increased toxicity was observed during its degradation while ECOSAR-predicted toxicity values of all identified intermediates were lower than that of fluoxetine, suggesting the formation of unidentified secondary by-products that contribute to the overall toxicity. The study showed that catalytic reduction is a promising remediation process for treating and defluorinating the fluorinated pharmaceutical compounds.

Sequential biowashing-biopile processes for remediation of crude oil contaminated soil in Kuwait.

The application of biological processes for remediation of the aged crude oil-contaminated soil of Kuwait can be an inefficient way, thus, this study developed 20 d-sequential biowashing and biopile processes where the biowashing step uses an enrichment culture of the indigenous soil bacterial community and the biopile step includes hemoglobin-catalyzed oxidation (HCO). The residual total petroleum hydrocarbons (TPH) concentrations and CO2 generation were measured to determine the removal efficiency, and the bacterial community changes were studied to investigate the effect of the sequential processes on the soil indigenous bacterial community. The enrichment culture grown on hemoglobin showed an increased surface activity, and this promoted desorption and emulsification of crude oil from the soil sample in the biowashing step resulting in 75% TPH removal. Potential surfactant-producing bacterial species were observed in the soil sample after biowashing. The HCO in the beginning of the biopile step removed 21% of the residual TPH, and further TPH removal was observed with a longer biopile period. Overall, the sequential biowashing and biopile processes removed 86% TPH. The results show that the developed sequential biowashing and biopile processes can be used to efficiently remediate the aged crude oil-contaminated soil of Kuwait.

Interaction among soil physicochemical properties, bacterial community structure, and arsenic contamination: Clay-induced change in long-term arsenic contaminated soils.

Pyrosequencing analyses to determine soil bacterial communities were conducted with forty-two soil samples collected from rice paddy and forest/farmland soils (Group A and B, respectively) at a long-term As-contaminated site. Soil physicochemical properties, such as the concentrations of As, Fe, Al, and Mn, pH, organic matter content, and clay content, were found to be significantly different with land use, and more importantly, strongly affected the bacterial community structure of the soil samples. When fitting the soil properties onto a nonmetric multidimensional scale plot of soil bacterial communities, clay content was found to be the most important factor in clustering the bacterial communities (R2 = 0.4831, p-value = 0.001). Phylum Chloroflexi (-1.03 of bioplot score) and Planctomycetes (1.31 of bioplot score) showed a significant relationship with clay content in soil samples. Interestingly, thebacterial phylotypes linked to clay content were only found in the soil samples of group B with low clay content, and had a strong relationship to As contamination in the redundancy analysis and the correlation analysis.Our results suggest that clay content seems to be negatively related to As contamination in soils, which, in turn, strongly influences the structure of bacterial communities in As-contaminated soil.

Effect of initial pH, operating temperature, and dissolved oxygen concentrations on performance of pyrite-fuel cells in the presence of Acidithiobacillus ferrooxidans.

Fuel cell technology can be applied to remove pyrite from pyrite containing mine waste (PCMW) and to generate electricity. This study investigated the effect of pH, presence of Acidithiobacillus ferrooxidans, operating temperature, and dissolved oxygen (DO) concentration on the performance of pyrite-fuel cells (PFCs). These factors affect the pyrite dissolution rate, which affects the electron movement for electricity generation, hence electrical performance. The PFCs performance based on the maximum power density and maximum current density, obtained on the 28th day, was better at pH 2.2 (0.74 mW m-2, 28 mA m-2) than at pH 4.3 and pH 6.5 and in the presence of A. ferrooxidans (i.e., biotic PFCs) than in the abiotic PFCs. The biotic PFCs showed more consistent performance regardless of the operating temperature than the abiotic PFCs. The PFCs performance was better at higher DO concentrations (30-33 mg L-1) than at lower DO concentrations (8-9 and 0-2 mg L-1); however, gas purging used to adjust DO concentrations could adversely affect the biotic PFCs performance. This study demonstrates that PCMW treatment and electricity generation can be achieved using the fuel cell-based technology, and the PFCs performance can be optimized by adjusting the operating conditions.

Heavy metal and sulfate removal from sulfate-rich synthetic mine drainages using sulfate reducing bacteria.

The removals of heavy metals and sulfate in the synthetic acid mine drainages (AMDs) by Desulfovibrio desulfuricans, sulfate-reducing bacteria (SRB), and the indigenous bacteria isolated from the mine area soil sample were studied to compare the AMD treatment efficiencies. The AMD treatment by the D. desulfuricans grown in the Desulfovibrio medium was used to represent bioaugmentation, while the AMD treatment by the indigenous bacteria grown in the Desulfovibrio medium was used to represent biostimulation. The consumption of lactate and sulfate suggested that the zinc (Zn) removal in the Zn-spiked Desulfovibrio medium by D. desulfuricans involved chemical precipitation and biosorption. The complete Zn removal by D. desulfuricans took 24 h, while the indigenous bacteria took 360 h. The significantly lower rate can probably be attributed to the composition of the culture. The removal of Zn in the sulfate-rich synthetic AMD-containing Desulfovibrio medium (i.e., AMD) was adversely affected by the presence of other heavy metals. Also, the sulfate reduction by D. desulfuricans and the indigenous bacteria was reduced from 47% to 20% and from 36% to 6%, respectively. The inhibitive effects on the removal of heavy metals and sulfate were greater with the Zn/Cu-spiked AMD than the Zn-spiked AMD. Overall, the indigenous bacteria showed potential for removing heavy metals and sulfate in AMDs, while the removal efficiency was lower than D. desulfuricans. The continuous supply of carbon sources with an adaptation period may be required to enhance the AMD treatment efficiency by the indigenous bacteria.

Photodegradation of tetracycline and sulfathiazole individually and in mixtures.

Antibiotics in environment can be of concern as they can enter the food chain posing risks to ecosystems and human health. Photodegradation has been considered as a promising way of naturally degrading antibiotics in environment. Antibiotics are usually present in mixtures in environment; however, previous studies focused on individual compounds. Therefore, this study investigated the effect of UV irradiation on the degradation of tetracycline (TC) and sulfathiazole (STH) in individual solutions and mixtures. Under dark conditions, the initial masses of TC and STH were reduced by about 35% and 26%, respectively, over a 35 d-reaction period. With UV irradiation TC and STH were completely removed within 14 d and 35 d, respectively, regardless of the initial concentrations. Both the TC and STH removals were faster (i.e., 2-4 times) when they were in mixtures. This may be partly attributed to the byproducts such as sulfate that can promote indirect photolysis and partly to the enhanced hydrolysis due to changes in the solution pH. Overall, this study suggests that when photodegradation is used to remove antibiotics in water, the removal kinetics of antibiotics individually and in mixtures can be considered to develop more efficient treatment technologies.

Role of hemoglobin in hemoglobin-based remediation of the crude oil-contaminated soil.

This study investigated the changes in the indigenous microbial community structure with hemoglobin (Hb) application to determine the role of Hb in Hb-based remediation of crude oil-contaminated soil. The phylogenetic diversity of the bacterial community showed that the Hb addition selected surfactants-producing species, thereby, promoting TPH degradation. The significant increase in the CO2 generation, which can be related to the increase in the bacterial abundance inferred from the 16S rRNA gene copy number, supports the enhanced TPH degradation with Hb application. The similar residual TPH concentrations in the presence of only hydrogen peroxide (H2O2) and both Hb and H2O2 suggested that the role of Hb as a catalyst was not as significant as the role of Hb as a nutrient. Also, in the presence of H2O2, a greater recovery of the microbial community structure was observed with the double Hb injection than the single Hb injection. Overall, this study shows that the Hb-based remediation strategies via microbial metabolism can be successfully applied to remediate the crude-oil contaminated Kuwaiti soil.

Optimization of hydrogen peroxide-to-hemoglobin ratio for biocatalytic mineralization of polycyclic aromatic hydrocarbons (PAHs)-contaminated soils.

This study investigates the efficiency of hemoglobin (Hb)-catalyzed biocatalytic reactions for removal of polycyclic aromatic hydrocarbons (PAHs) in a historically PAHs-contaminated soil and of benzo(a)pyrene (BaP) in an artificially BaP-contaminated soil. PAHs removal tests at various H2O2-to-Hb mass ratios (0-3.7) showed that the PAHs removal was greater at H2O2-to-Hb mass ratio of ≥3. This was attributed to the greater removal of high molecular weight PAHs at higher H2O2-to-Hb mass ratios. The BaP removal increased from 36% to 85% with increasing H2O2-to-Hb mass ratio from 1 to 3, and further increase in H2O2-to-Hb mass ratio decreased the BaP removal. Thus, the optimal H2O2-to-Hb mass ratio for BaP removal was determined to be 3 in the artificially BaP-contaminated soil. The BaP removal in the presence of only Hb can be attributed to the capture of BaP by Hb. The increased BaP removal in the presence of H2O2 is likely due to BaP mineralization as the BaP removal and the CO2 generated showed a strong positive correlation (R2 = 0.999). Overall, this study shows that the Hb-catalyzed biocatalytic reactions can effectively remove PAHs in soil.